Jiawu Zhao

2 Questions 3 Answers 0 Followers

Questions related from Jiawu Zhao

To stimulate T lymphocyte, people use either soluble anti-CD3 or immobilized anti-CD3. May I ask what is the difference between them?

It seems that immobilized anti-CD3 has stronger stimulant effect.

27 September 2012 1,794 40 View

To stain for cytokines in lymphocytes (e.g. IFN-g), are simultaneous stimulation and Golgi block essential?

I want to use an antibody to stain IFN-g in lymphocyte. After initial activation for 2 days, is it essential to add an extra stimulus (e.g. anti-CD3e) when I add in Golgi stop?

28 May 2012 2,329 11 View