Jiahao Ye

10 Questions 18 Answers 0 Followers

Questions related from Jiahao Ye

Will conjugated saporin also affect passing axons or long-range projection neurons?

I have this question just out of curiosity. I read a couple of publications in which a saporin-conjugate is used to kill a specific neuron population, for example GABAergic neurons by stereotaxic...

07 July 2016 2,593 3 View

How is that paper continues getting cited THREE years after the retraction?

It is not rare that an unfortunate biology paper has to be retracted. Such is the case for a 2011 Journal of Neuroscience paper (http://www.ncbi.nlm.nih.gov/pubmed/21900561) which was retracted in...

08 August 2015 8,157 4 View

How do you minimize the background staining of c-fos, Arc etc. in C57BL/6 mice?

C57BL/6 mice seems to be easily stressed during the daily handling and possible transfering during behavior test. How do you do to decrease the amount of IEG induction in rodent brain which are...

06 June 2015 9,240 7 View

How can I minimize the precipitation of fluorogold in tract-tracing experiments?

In fact, I am have a good struggling with coinjection of fluorogold &BDA in the same brain region. My protocol for this combination is quite similar to which L. Swanson reported in PNAS, 2010...

05 May 2015 10,023 6 View

Do neuronal activity markers like c-fos, Arc distinguish between elevated synaptic activity and elevated intrinsic neuronal activity?

The question is based on the wide use of neuronal activity markers including Arc to dissect neuronal circuits activated in certain behavior or to certain stimuli. Does anyone have any idea of...

04 April 2015 9,161 19 View

How should one prepare formalin solution for formalin test in mice?

Low concentration (e.g. 2% to 5%) of formalin solution is widely used for well-established formalin pain test in rodents. But can I use the same as the formalin solution for fixative (such as the...

02 February 2015 2,913 5 View

How do you judge a research paper?

For the peer-reviewed papers, how do you judge the quality of a research paper based on its scientific merit?

10 October 2014 5,484 0 View

Does anyone have experience on transverse brain stem slices with MNTB?

I plan to record the synaptic transmission at the Calyx of held for the first time. But I find it quite difficult to get a right transverse slice. I dissected out the brain stem, but could rarely...

06 June 2013 7,582 3 View

Why overexpress PSD-95 increases mEPSC frequency but not amplitude?

PSD-95 is postsynaptic protein, so why it has major impact on mEPSC frequency for which presynaptic structures are expected to be responsible? Strangely enough, overexpression do not enhance...

12 December 2012 4,287 6 View

How to dissect out the neuronal connection within specific areas in small brains, such as flies, zebrafish, insects?

Because brains are extremely small in same species, so normally a small volume of brain may contains many areas, probably the connected ones. When there is no available genetic line, it is usually...

12 December 2012 5,342 1 View