Jiafen Hu

2 Questions 5 Answers 0 Followers

Questions related from Jiafen Hu

Does anybody have a protocol to share on reducing or eliminating autofluorescence in frozen tissues?

We are trying to label T cells in tissues with fluorescently-tagged antibody and find very high autofluorescence background present in tissues such as spleen. We used acetone to fix the frozen...

09 December 2013 1,757 0 View

Does anyone have experience in culturing rabbit cytotoxic lymphocytes (CTLs) in vitro?

We have a rabbit model system to develop vaccines. Because of the limitation of reagents for rabbit T cell culture, we have to optimize our in vitro culture conditions all the time. Nonspecific T...

24 October 2012 7,265 3 View