Jia Wen-juan

5 Questions 2 Answers 0 Followers

Questions related from Jia Wen-juan

How to isolate human primary monocytes from PBMC by herence?

We are trying to isolate primary human monocytes from peripheral blood. Following PBMC isolation with Ficoll, we want to enrich monocytes by herence. We culture PBMC with serum-free 1640 for 2-24...

05 January 2023 2,868 3 View

How should I design the N-terminal VC plasmid of BiFC assay?

I'm going to detect protein-protein interaction with BiFC assays and my target proteins are ER retention with KDEL motif. so I want to fuse VC to the N-terminal of my protein. My question is if...

23 July 2020 3,598 1 View

Why neonatal lethality of knockout mice decreases by degrees during mating?

I observed the neonatal lethality of knockout mice( homozygous ) is almost 75% after the knockout mice was generated. But during matings between heterozygous mice to produce offsprings, I...

29 October 2019 2,786 0 View

How to count cells in different slide stained with different antibodies and calculate percentage ?naked eye or software,which software?

Immunofluorescence assay, my target protein locates both in ER and Golgi, I want to calculate the percentage in the two organelle.

15 June 2017 7,633 2 View

The endogenous localization of my protein is ER, while the stable transfection to 293 T or CHO-K1 is golgi,why?

the protein expresses specifically in brain and lung.

08 June 2017 4,974 4 View