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Questions related from Jesus Antonio Aldana-Mendoza
Hello everyone, today I ran my ellman's reagent twice using a 96 well plate and tried to read the concentrations of my standards of my L-cysteine using a elisa plate reader and reading the...
13 July 2017 4,009 1 View
Hello Everyone, the other day I tried to run a ellman's regent experiment using L-cysteine as my control. the overall objective was to plot the values obtained from the standards(L-cysteine) to...
26 June 2017 7,768 6 View
Does anyone have a protocol for ellman's regent test. I was looking at some protocols and one article mentioned using Cysteine Hydrochloride Monohydrate as a standard control. the article also...
26 April 2017 435 3 View
Hello everyone, I am trying to see if my protein has irreversible, reversible, or it forms disulfide bonds. Previously, I mixed my protein ( 25uM) with MAL-PEG 5000 ( 250 uM) to see if the...
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