Jesse Gerard Meyer

4 Questions 9 Answers 0 Followers

Questions related from Jesse Gerard Meyer

Is the Pacbio Sequel or the Illumina HiSeq 2500 better for detection of alternative splicing by RNA-seq (next-generation sequencing, NGS)?

We have a biological condition that we believe decreases splicing fidelity in c elegans. In our preliminary RNA seq data using 2x50 bp, 22 million reads per sample and 5 replicates, we found only...

29 March 2018 3,292 3 View

Has anyone compared zero air with nitrogen for nanoflow electrospray of peptides using 5600 with NanoSpray III source?

We are spending more than the cost of the service contract every year on buying 'zero air' cylinders for the electrospray sheath gas. We have the 5600 and 6600 with NanoSpray III sources. We...

01 November 2017 4,376 3 View

What is the best way to quantitatively esterify a mixture of peptides, for example, with heavy and light methanol to quantify differences?

I think I read some phosphoproteomics papers in the late 2000s where people would quantify differences in enriched phosphopeptides using differential isotopic labeling of acids with esterification...

24 March 2016 2,593 3 View

How does increased orbitrap resolving power change signal abundance?

I had a recent discussion about orbitrap signal as a function of resolving power setting . My understanding of the orbitrap detection is that a fixed packet of ions, as determined by the...

12 April 2014 1,464 4 View