Jeremy C. Borniger

4 Questions 16 Answers 0 Followers

Questions related from Jeremy C. Borniger

Multiple peaks in EEG FFT. What's that about?

Hi all, I'm scoring some sleep data and noticed multiple (maybe harmonic) peaks in the EEG FFT (denoted with arrows) at some points during the recording. Can anyone explain why this phenomenon...

05 May 2017 8,301 6 View

Is there a standardized test for pain (nociception) in rodents?

I'm trying to determine pain levels in mice (not allodynia or hyperalgesia, but regular pain). As pain is rather subjective, it is hard to quantify, especially in rodents. What are the most...

28 December 2016 4,578 7 View

Why do I get no c-fos labelling in my immunofluorescence experiment?

Hi all, I'm trying to co-label orexin and c-fos. I can get orexin neurons to label very well with the following protocol, however I get no visible c-fos labeling (I've tried 3 different...

03 July 2015 7,553 36 View

What is the best way to test humoral immune function in cell culture supernatant from Siberian hamsters (Phodopus sungorus)?

I have supernatant from LPS stimulated splenocytes and lymph node cells. Originally I planned on using ELISA to measure specific cytokines, however the antibodies I've been using (from mouse,...

15 June 2015 612 8 View