Jeremie Nestor

3 Questions 1 Answers 0 Followers

Questions related from Jeremie Nestor

Primary epidermal human melanocyte culture?

Hi everyone, I started culturing primary human melanocytes and am facing some problems. They grow very slowly, and after 15 days there seems to be no more growth (50% confluence). I follow...

05 August 2022 8,835 0 View

Precipitate in cell supernatant after freeze/thaw cycles, is it normal?

Dear All, At the end of a cell culture experiment, I collected the supernatant and centrifuged it, and did not notice any precipitate. After freezing the supernatant, thawing and centrifuging...

10 December 2021 6,332 3 View

Why some Elisa kit are not suitable to determine proteins in cell culture supernatant?

I want to use an ELISA kit based on competitive evaluation, and in the documentation of the kit they mention "influenced by the factors including cell viability, cell number or sampling time,...

01 September 2021 4,637 3 View