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Questions related from Jelle Bonthuis
In the pFRT/LacZeo plasmid, there is an SV40 small T intron downstream of the last cDNA but before the SV40 poly A signal. What is the function of this intron? Should I remove this intron when...
27 September 2021 8,008 1 View
I would like to develop a lentiviral delivery plasmid that contains two transgenes with independent promoters. I have two main questions regarding the design: The specific promoters are CMV and...
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As I understand (on a quite basal level), adipocytes (specifically White adipose tissue (WAT)) absorb fatty acids in an insulin-dependent manner. How do insulin-resistant adipocytes absorb as much...
13 February 2017 2,913 3 View
Different logical approaches based on existing literature produce two contradicting hypotheses. My initial hypothesis was that downregulation of CB1 receptors by cannabis use would increase cell...
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I was under the impression that CUT&RUN's benefits came primarily from reducing cell input count. But with (not so recent) improvements in ChIP such as Nano-ChIP and LinDa-Chip, the latter of...
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