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Questions related from Jeffrey R. Kelly
I am currently attempting to do a double-label stain for Phosopho-S6 protein and Vasotocin in zebrafish brains. I have been able to get each antibody to work properly independently, but the...
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So I did a test run of my 5HT staining today and found that at dilutions of 1:10,000, 1:25,000, 1:5,000, and 1:2,500 I did not have proper fluorescence of my cells with 5HT in them. When zooming...
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I will be doing a 5HT immunohistochemical procedure on Long-Evans rat brains in the near future and am following a procedure that implements primary and secondary antibodies for staining...
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