Jeff Falcone

4 Questions 12 Answers 0 Followers

Questions related from Jeff Falcone

How to purify large amounts of plasmid after restriction digest?

I'm trying to linearize large amounts of plasmid (500 µg - 1000 µg). I can easily digest this amount of plasmid, but when I precipitate with ethanol and resuspend in TE, I'm left with a milky...

11 November 2019 3,030 5 View

Does anyone know where I can find an image of cilia in live neurons?

I'm looking for an image of primary cilia in live neurons. I've seen many images of cilia in fixed neurons, but I can't find an image of a live neuron with a primary cilium.

03 March 2018 2,404 3 View

Cell line with endogenously expressed gfp tagged protein?

Hello, I need to get a rough idea of what a gfp-tagged protein looks like at endogenous expression levels as opposed to overexpressed. The protein that gfp is conjugated to is irrelevant for my...

10 October 2016 2,524 2 View

Does anybody have a good antibody for staining basal bodies of primary cilia?

Hello, I'm trying to costain cilia with acetylated-alpha-tubulin antibody (6-11b-1 mouse monoclonal) and something to mark the base of the cilium. My first attempt was with Anti-gamma-Tubulin...

01 January 1970 952 14 View