Jaymin J Kathiriya

2 Questions 2 Answers 0 Followers

Questions related from Jaymin J Kathiriya

What is the best way to fix and embed cells/organoids growing in a 3D matrigel?

We have been growing cells in 3D organoids (1:1 matrigel/growth media mixture) in a transwell insert. However, when we try to fix the cells with 3.7% PFA, the matrigel becomes a little loose and...

18 December 2017 8,757 3 View

Can anyone share their Hydroxyproline Assay protocol not utilizing any kits?

I am planning to perform a hydroxyproline assay on fibrotic lung tissues. We are not planning to purchase any kits and wanted to know if someone has been using their own protocol for measuring the...

13 June 2016 5,590 2 View