Jayita Barua

2 Questions 1 Answers 0 Followers

Questions related from Jayita Barua

Mouse MSCs not expressing TSG-6 on cytokine stimulation?

I am using mouse bone marrow derived MSCs (passgae 3-6) for my in vitro cytokine priming experiment. My basal media is 2% FBS-DMEM. I am using 10 ng/ml TNF-a, or IFN-g or both for 24 hours. I see...

28 May 2024 8,890 0 View

How neutrophil isolation on ice affect the cells and culturing subsequently?

I am using a magnetic separation based isolation kit for mouse neutrophils and the protocol mentions 2 incubation on ice for 15 min each. I am aware that low temperature pre-activate the...

06 September 2023 6,270 0 View