Jaya Gosala

4 Questions 26 Answers 0 Followers

Questions related from Jaya Gosala

ELISA negative control which is without coating giving high background, not explaining the logic?

I’m using ELISA method : Coating (antigen) > Blocking > sample/standard (antibody) > secondary antibody ( HRP conjugated)> TMB substrate Negative controls: · No Coating (PBS)...

06 June 2016 9,052 13 View

Why weren't BHK-21 cells growing in Serum free medium for recombinant protein expression?

I was trying to adapt BHK-21(C-13) cells to serum free conditions. Cells were initially cultured with 10% FBS and I was trying to intervene the serum. For this I used Excel CD 302 medium, VP-SFM...

09 September 2014 4,983 5 View

Transformation and Ligation

I'm trying to clone a 3.0Kb insert into a 5.5Kb vector backbone. Both insert and vector showed expected sizes before the ligation setup. After transformation, I got more colonies in the control...

12 December 2012 6,988 32 View

Why do I have good colony difference after ligation, but no recombinants?

I'm trying to clone 3.0 Kb inserted into 5.5 Kb vector with one side that is blunt and one side that is a sticky end. After transformation, 20 times colony difference was observed between test and...

12 December 2012 354 6 View