Jatuphon Touché Nakhonsri

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How do you prepare mitochondria from mammalian cells (eg. LCL, fibroblast) for oligomycin sensitive ATPase activity assay?

I'm setting up oligomycin sensitive activity assay by using spectrophotometry to observe decrease of OD 340 which represent usage of NADH. In the experiment, I assayed (1) solubilized...

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