I want to test my sample with a carrier DNA (stock 1mg/ml). How much of this do I have to add to make it a working solution of 500ng in my 20ul PCR ?

I am going to do a nested PCR. and also test with a carrier DNA (salmon sperm DNA stock is 1mg/ml). The protocol suggests using 500ng in the reaction mixture. Do I make a diluted stock from the...

23 March 2021 7,466 3 View