Jason Tan Liwag

4 Questions 2 Answers 0 Followers

Questions related from Jason Tan Liwag

Problems with mammosphere generation of MCF7 lines?

I'm generating mammospheres from MCF7 lines in phenol-red free DMEM/F12, 20 ng/mL of EGF, 10 ng/mL of bFGF and 1X ITS-A (Gibco; 51300-044) on Nunclon Sphera 24-Well Plate. And before, I'd see...

05 June 2019 2,751 0 View

Do you know of any tumor suppressor genes that are upregulated in cancer cells that are metastatic/highly aggressive?

Tumor suppressor genes (such as Rb, APC, PTEN and the ever famous p53) are often downregulated in cancer cells, if not completely lost or dysfunctional. I'd like to know if there are any tumor...

07 March 2017 8,034 3 View

What is the best/ simplest protocol for counting the cells when culturing the breast cancer cell lines?

I am currently pursuing a study regarding breast cancer cell lines. I'm looking for best/ simplest protocol for counting the cells. Also, could you suggest machinery that would be necessary for...

15 March 2015 5,997 5 View

What are the most appropriate cell assays that can be done for cancer cells?

I'm planning to expose cancer cells to a certain concentration of a substance and am primarily looking to observe its mitotic activity. Does anyone have any suggestions as to what cell assay I can...

15 December 2014 948 7 View