Jason Edward Hammonds

3 Questions 11 Answers 0 Followers

Questions related from Jason Edward Hammonds

Can someone suggest an Ab that will positively identify human CD4 T cells in macrophage co-culture experiments by IF?

Tried a few against hCD4, but they did not discriminate between CD4+ T cells and primary human macrophages. Both about equally as bright. Expected the T cells to be much brighter. Thought of...

02 September 2015 2,155 8 View

How best to poly-D-lysine coat tissue culture dishes for primary hMDM adherence?

I think I can do better than I currently am.

23 October 2013 3,862 7 View

What is the life-span of human macrophages in culture? What is the optimal experimental window and should GM-CSF be excluded once cells have differentiated?

Cells were enriched by negative MACS selection. Differentiated in growth media supplemented with 5 ng/ml GM-CSF. GM-CSF included throughout feedings. Cells seemed to crash around day 14, or rather...

12 July 2013 9,986 11 View