Janna Bashar

4 Questions 8 Answers 0 Followers

Questions related from Janna Bashar

How to increase yield for thioglycollate elicited peritoneal macrophages?

Hello, I am learning how to isolate thioglcollate primed peritoneal macrophages from mice but my yield has been lower than expected. I expect a total yield of 1x10^7 but I have had inconsistent...

23 February 2021 2,177 4 View

How could I determine the starting methyltransferase enzyme and methyl donor to methylate a synthetic peptide?

Hello, I have a synthetic peptide with arginine residue I aim to methylate using PRMT1 + SAM (methyl donor). I then will use my dimethylated arginine peptide product in ELISAs and Western blots....

13 November 2020 5,745 7 View

ELISA-Why does my diluted high absorbance sample have such lower concentration compared to less diluted sample when using standard curve?

Hello! Our lab has developed a standard curve with known concentration that we use to determine our ELISA concentrations. We then multiply by the dilution factor for our final concentration....

26 October 2020 8,821 6 View

Protocol for whole heart tissue lysis for Westerns?

Hello, I am troubleshooting a western protocol using whole heart homogenates but I seem to be getting consistent smearing at the lower band range of my gel which is also where I want to see my...

02 October 2019 8,877 4 View