Jana Frank

6 Questions 9 Answers 0 Followers

Questions related from Jana Frank

Plasmid rearrangement in E. Coli?

Hi Just wandering if anyone else is observing a rearrangement of a plasmid, with partial loss of the plasmid region that doesn't contain Ori, in plasmids that contain two or more of the...

10 March 2023 8,273 5 View

Problems with BAC DNA isolation. ?

Hi, I`ve been trying to isolate genomic library containing 160 kb BAC DNA using Nucleobond Xtra Midi, Vivogene BAC kit, Jetstar and a column-free method using Qiagene`s P1, P2 and P3 buffers....

14 December 2017 7,023 2 View

CRISPR/Cas9 KO homology arm under 500 bp?

Hi I want to KO a gene and for this I am targeting the exon 1 of the gene. To increase the frequency of non-specific KO events (gRNA has six one bp mismatches in the first 13 bp) I am designing a...

08 February 2016 3,828 3 View

Anyone familiar with G-banding (DNA) free software?

Hi We are doing some G-banding but it is, for now, a one-time experiment, therefore we would like to avoid buying software for the banding analysis. Does anyone know of a free G-banding analysis...

15 June 2015 2,862 1 View

Is anyone familiar with metaphase FISH biotin detection?

Hi, I have a question regarding the detection of biotinilated probe in metaphase FISH, using old slides (made mid July 2014). I use DIG~ and Biotin-Nick mixes to generate the probes. I try to...

08 October 2014 9,830 1 View

What is the best way for a long term storage of FISH sides for metaphase FISH? The slides have been on ambient T for one month due to transportation.

The slides were prepared a month ago and were shipped to me with a delay. During the shipping, and I assume also during the storage, they were maintained at ambient temperature (laboratory,...

22 August 2014 1,025 5 View