James Vincent Vowles

2 Questions 4 Answers 0 Followers

Questions related from James Vincent Vowles

Do localization tags (NLS specifically) affect protein function or steady state expression levels?

I have found that tagging a protein with an NLS decreases the expression level by a factor of 2 or more. When comparing tagged and untagged constructs in HEK293 the NLS shows lower protein levels...

05 May 2014 1,950 4 View

What is the volume of a HEK293 cell?

Does anyone know the volume of a HEK293 cell? I'm also looking for the volume of the nucleus. My literature searches (mostly through BioNumbers) have turned up a range of HeLa cell volumes...

11 November 2013 7,457 6 View