James Rooney

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Questions related from James Rooney

Is there any issue with my gene/plasmid construct?

I have tried expressing 3 different proteins in yeast, all are different sizes, 8kDa, 11kDa and 15kDa. However, when I run the expressed proteins on a 4-12% SDS-PAGE they all appear as the same...

14 October 2022 7,858 1 View

Different proteins (each a different size) are appearing as same size on SDS-PAGE?

Hello all! I have completed a test yeast expression for 5 different proteins, and I have taken a sample of the culture at 0, 24, 48 and 72 hours. 3 of my yeast cultures seemingly expressed a...

12 October 2022 3,319 5 View