James David Londino

2 Questions 12 Answers 0 Followers

Questions related from James David Londino

Easy way to measure LacZ expression

I want to measure LacZ expression from an adenoviral vector in epithelial cells. I don't need anything particularly quantitative since I only want a yes or no answer as far as expression is...

03 March 2013 1,142 5 View

Are there any tricks to getting 16HBE cells to polarize quickly in a permeable support?

I am trying to get my cells to polarize as quickly as possible after transfection so i can measure differences in current via Ussing chamber. What density do you seed a 6.5mm filter? What coating...

03 March 2012 3,813 0 View