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I'm currently preparing for some in situ hybridisation, which requires Maleic Acid Buffer with Tween (MABT) for some of the wash steps. The recipe i have been given is for 1L of 5x concentrated...
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I ran a gel yesterday, and for some reason the running buffer was extremely hot after one and a half hours of running the gel. I feel like this shouldn't be allowed to happen but am I right? I...
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I am a student in my final few weeks of a summer research project. I am working on MIN6 cells which I am currently culturing, however as of the start of the week they appear to be struggling to...
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I am a student currently working on a summer project involving MIN6 cells. Part of my project involves measuring ER stress, which means I have to do many Western Blots. However, I am coming to the...
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I am a student researcher, investigating whether glibenclamide can restore insulin secretion in cells with Wolfram syndrome (WFS1 knockdown). The team I am working with have been gifted some MIN6...
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