James P. Madigan

5 Questions 11 Answers 0 Followers

Questions related from James P. Madigan

Can I package a MSCV retroviral construct using the pCL10A1 retroviral packaging plasmid?

Hi! I want to make retrovirus from a MSCV retroviral construct, for use in making a stable cell line. I was wondering whether anyone knows if I can use the pCL10A1 retroviral packaging plasmid? I...

06 October 2021 3,519 0 View

Does anyone know the staus of Akt phosphorylation in HepG2 cells?

Meaning, is there a loss of PTEN and/or an increase in upstream signaling to PI3K/Akt that would lead to above average phospho-Akt levels, as compared to other cell lines? Thanks y'all!

15 February 2017 7,619 2 View

What are some commonly used glioblastoma (GBM) cell lines that people use?

I would like to do some signaling studies in GBM cell lines. Might someone suggest some commonly used ones? Thank you!

17 May 2016 9,518 3 View

Has anyone used a colorometric or fluorometric kit to measure free fatty acids in cell culture cells?

I want to measure possible changes in fatty acids in various cancer cells in culture after modulating the expression of a certain protein? I don't have the $ or the resources to do this...

04 May 2016 9,943 1 View

Anyone successfully use the cross linker DSP?

I am trying to enrich for an unstable, potential protein-protein interaction. I've read several papers that have used the chemical crosslinker, DSP. Has anyone successfully used this reagent? If...

16 January 2015 5,927 6 View