What can cause excess precipitate when purifying protein with TCA?

I had eluted some protein from plants from magnetic anti-Strep tag beads with 1.8ml of buffer with biotin (100 mM NaPhosphate, pH 8.0; 150 mM NaCl; 0.05% TX-100; 50 mM Biotin). When I added 100%...

02 July 2020 4,289 7 View