Jakub Began

2 Questions 2 Answers 0 Followers

Questions related from Jakub Began

What is happening during the ITC titration? Atypical dataset?

Dear colleagues, I am wondering how to interprete titration (attached) of my soluble protein (50 kDa) with its highly polar LMW ligand (amide and sulfonic acid groups, 1.4 kDa). TItration was...

27 August 2020 3,459 5 View

Any experience with secretion of protein from periplasm into the media or directed sequence-based protein degradation in periplasm of E.coli?

I have been thinking of a construct, which is single-inner membrane-spanning protein, the N-terminal domain of which I want to get rid off after enzymatic activity I will induce in the E.coli...

16 November 2015 3,546 0 View