Jahnvi Ahlawat

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Questions related from Jahnvi Ahlawat

Which method is more effective for endotoxin removal : Triton X-114 phase separation or endotoxin removal columns?

I am working with a recombinant protein that requires efficient endotoxin removal for downstream applications. I am considering using Triton X-114 phase separation or endotoxin removal columns....

27 February 2025 2,377 5 View

How to detect the Refolding efficiency of a denatured soluble protein?

I am a M.Sc. student. I have successfully expressed my protein in soluble part using a solubilizing tag. I faced problems in Ni-NTA purification, as protein was not binding to the column. So, I...

13 June 2023 2,927 4 View