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Hi, Can anyone suggest me how to get NHEt in the C terminal of peptide during SSPS? I cannot do post synthesis selective modification as the peptide has R and K in the sequence? Should I use any...
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I want to conjugate an oligonucleotide with a peptide via N terminal and 3' amine of the the peptide and oligonucleotide respectively. Requesting your valuable suggestions in this.
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Hi, I am measuring the absorbance of Cu2+ in solution in a scan mode in CARY 60, Agilent. Absorption maxima of Cu2+ in solution is 800nm. I want to know if I can use conventional quartz cuvette...
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I want to check cell internalization of Abeta (1-42) peptide w.r.t. However, I do not have fluorescent labeled Abeta peptide to perform this experiment. Is there any other ways by which I can...
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Qualitative analysis of amyloid formation can be monitored by ThT fluorescence study(Ex:440nm and Em: 485nm). Usually, at a given time, I record the Emission spectra of the sample thrice and...
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I am synthesizing a peptide that contains few D- amino acid residues in between. Now that I have completed my synthesis, I would like to verify if the D-residues are incorporated in the same place...
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Does 1,1,1,3,3,3-Hexafluoro-2-propanol(hfip) react with polypropylene that is being used in making MCTs? i have been getting unknown particulate contaminations in my sample after treating with...
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My work requires gel electrophoresis of some truncated peptides aggregates (less then 10 residues long containing aromatic aa in between) in their native form. I cannot opt for using SDS because...
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