Jaeyoung Park

2 Questions 3 Answers 0 Followers

Questions related from Jaeyoung Park

What cause protein aggregation?

Recombinant proteins extracted from E. coli were purified twice using a Ni-NTA column and an antibody-coupled agarose bead column. After final purification, the protein is in a 10% solution of 1M...

24 August 2023 8,585 2 View

Can bacterial lysate pass through 0.45um cellulose accetate syringe filter?

When lysing E. coli for recombinant protein expression, I utilized a lysis buffer composed of 1M Tris-HCl, Triton X-100, glycerol, lysozyme, MgCl2, and a protease inhibitor cocktail. After lysing...

15 August 2023 8,428 4 View