Jacob Keller

3 Questions 4 Answers 0 Followers

Questions related from Jacob Keller

Does anyone know of a cell line or easily-accessible system in which glycogen stores can be perturbed?

I am looking for an easily-transfectable cell line whose glycogen content can be easily perturbed by adding, for example, epinephrine or insulin or something. Is there such a thing out there?

07 July 2017 9,318 1 View

For perfusion of bicarb containing / CO2-gassed buffers, are there alternatives to Tygon, which seems to be quite permeable to CO2?

I've noticed whilst measuring intracellular pH changes under various conditions that when bicarb/gassed buffers sit in the tubes, there is an initial artifactual pH rise in the cells until the...

06 June 2017 1,056 7 View

Anyone know of a robust insulin-response glucose-uptaking cell line?

I am searching for a cell line that is easily transfectable and reliably responsive to insulin stimulation as measured by glucose uptake, with a good protocol for evoking the insulin response. On...

05 May 2014 6,138 4 View