10 Questions 2 Answers 0 Followers
Questions related from Jacob Kurdys
Due to a low miRNA expression, I would like to preamplify our samples. I currently have taqman reagents, and taqman miRNA primers. Could I use the qaigen MiScript PreAmp PCR kit for this using...
14 September 2018 1,523 3 View
Cardiac cells were incubated in various experimental conditions. Cells were stained with fluorescent mitotracker and nuclear dye. I am having difficulty quantifying mitochondria from these...
19 June 2017 9,901 3 View
Our lab has sent rat cardiac tissue for sequencing and have obtained indigestible fastq data files. Is there a software I can use to organize these fastq sequence files in order to...
28 April 2017 7,891 3 View
This will be long, but I am out of ideas and require help. I am having difficulty with my real time PCR as I am obtaining multiple products in each sample. I am looking for expression of JDP2 in...
17 January 2017 9,896 6 View
I have isolated RNA from keratinocyte cells using Trireagent from SigmaAldrich. With varying concentration, low 260/230, and a spectra that matches phenol contamination, I suspect.......
20 October 2016 6,971 2 View
Which fixative PFA/Methonal/Acetone will preserve the myotube structure of cardiac rat Cells for immunostaining. I've use 4% PFA several times and it detaches about half of the cells, while...
11 August 2016 5,553 3 View
I am having difficulty finding an online program to create a gene regulation map. I have mapped the entire pathway by hand, but would like a presentable version for an article/poster.
25 May 2016 5,179 1 View
I am isolating RNA from rat serum using the Ambion MirVana kit. Analysis using Nanodrop shows my 260/280 is positive ~2.0, however my 260/230 is very low. Also, I cannot obtain a concentration...
04 April 2016 3,234 5 View
Human cells were analyzed via MTT (abs. 570nm) assay before being lysed in 10%SDS and frozen. Will I be able to determine protein concentration in order to perform a western blot? Will the SDS...
23 March 2015 3,344 4 View
I will be transporting HEKa cells for irradiation 90 minutes away and back. I will be using CO2 independent media from life technologies in 50mL conical tubes. At what temperature should I...
10 October 2014 6,504 3 View
