Jacci Newel

6 Questions 10 Answers 0 Followers

Questions related from Jacci Newel

What is the optimal time to perfuse a rat with ice cold ACSF for slice patch-clamp electrophysiology?

I’ve been unhappy with my slice quality lately and I’m looking to improve slice health. I’ve tried various times/variations of simple extractions and also transcardial perfusions. I just can’t...

29 February 2020 9,274 3 View

Any tips on rat brain extractions for PFC whole cell patch clamp recordings?

Could anyone willing please share their favorite protocol for rat brain extractions for PFC whole cell patch clamp recordings? I did just fine with simple extractions in mice, but rat skulls are...

27 February 2020 3,222 0 View

When running cue-induced reinstatement after extinction from cocaine self-administration in rats, do you run the reinstatement alone?

Hello all! I'm learning the ropes of cocaine self-administration, extinction, and cue-induced reinstatement in rats. My current group is almost finished with extinction, and will be ready for...

18 January 2020 2,419 4 View

What dye is safe to add to the ACSF inside glass electrodes when recording in-vitro LFPs?

Hi all, I've been learning to record LTP in in-vitro mouse hippocampal slices on an LFP rig. I have trouble seeing the very fine glass electrode tip (I pull 2 megaohm electrodes on the pipette...

14 January 2019 8,417 1 View

Does anyone else do in-vitro LFP recordings on rodent hippocampal slices? What do you use for lighting?

Hello, all! I'm inducing/measuring LTP in in-vitro mouse brain slices. My stimulating electrode is supposed to be in CA3 and my recording electrode is supposed to be in CA1 of the hippocampus. I...

24 October 2018 302 5 View

How do I troubleshoot the following intermittent 50 hz noise scenario (extracellular recordings)?

Hello all, I'm attempting to do in-vitro mouse hippocampal LTP recordings on an LFP rig. I am new to electrophysiology (so please forgive my ignorance), and I was hired onto a project to figure...

17 October 2018 5,028 16 View