Izhak Kehat

2 Questions 1 Answers 0 Followers

Questions related from Izhak Kehat

Why do we get much lower titers of AAV6 relative to AAV9?

We use the same backbone and helper plasmid for both, and the only difference is the Rep-cap plasmid 2/6 vs 2/9 from addgene. We titer by qPCR with a standard curve. Is this a known phenomenon and...

24 May 2022 6,644 0 View

How can I avoid the aggregation of nuclei in the initial stage of 3C?

I'm working with the recently published Nature protocols 3C-Seq protocol on fibroblasts. After lysing the cells with the hypotonic buffer (with or without additional dounce step), I re-suspend the...

17 December 2013 6,586 8 View