Ivan Jakovlić

3 Questions 12 Answers 0 Followers

Questions related from Ivan Jakovlić

Hypoxic environment in fish stomach?

We are studying two parasitic species, one parasitising on the gills of fish, and the other in the stomach. While it appears obvious to us that the stomach environment should be hypoxic in...

10 October 2019 5,540 1 View

How do I calculate temporal relative changes in gene expression during embryonic development?

I am analyzing temporal changes in target gene(s) expression during embryonic and juvenile (fish) development using qPCR. Since I don't have a control group, the usual fold change calculation...

08 August 2015 9,558 7 View

Does anyone have experience in keeping F and R PCR primers mixed together in one tube?

Basically, I'm trying to save time: as I'm doing qPCR almost on a daily basis, I am wondering if there is any reason not to prepare a master mix of H2O, forward primer and reverse primer in one...

06 June 2015 9,409 9 View