Itziar Serna Martin

2 Questions 4 Answers 0 Followers

Questions related from Itziar Serna Martin

How do I get a halophilic protein into low salt without aggregation?

Hello, I am trying to find a buffer composition that contains low or no NaCl for a protein that is purified in 500mM NaCl. The reason for lowering the salt is trying to obtain good vitreous ice...

02 February 2016 8,037 4 View

How to remove a codon by site directed mutagenesis using Phusion polymerase of Pfu Turbo polymerase?

I am trying to remove a stop codon at the end of my sequence by site directed mutagenesis. I have designed complementary primers which don't include the codon using the Agilent Technologies...

10 October 2013 4,615 5 View