Irina Kologrivova

4 Questions 8 Answers 0 Followers

Questions related from Irina Kologrivova

Can I simultaneously measure concentration of cytokines in serum and culture medium with Multiplex Luminex assay?

Dear colleagues, We are planning to use Human XL Cytokine Base kit for evaluation of concentration of several cytokines. The plan was to measure concentration of cytokines both in blood serum, and...

21 February 2024 7,658 0 View

Possible problems with eNOS intracellular staining in circulating endothelial cells?

I am trying to set up a protocol to study circulating endothelial cells by flow cytometry. First approach was to identify CD45-CD146+ population and analyze expression of cell adhesion molecules...

29 January 2020 9,295 3 View

If I want to study effects of glucose transport inhibitor on functions of lymphocytes in vitro, how should I choose the right concentrations?

I found methods to study influence of different drugs on viability of tumor cell lines. In this case the common approach is to estimate EC50/IC50. Different concentrations are added to cells and...

25 October 2017 8,389 1 View

What is the best plate format to culture human PBMC?

Dear colleagues, I need to culture freshly isolated human PBMC to harvest medium for the subsequent determination of cytokines' concentration. Intact cell culture as well stimulation with LPS and...

16 November 2016 8,178 16 View