Iramis Rosique

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Questions related from Iramis Rosique

More than 500 ug of yield with maxi plasmid kit of qiagen. Is that possible?

The company says 500 ug is the max yield for maxi kit, but in my lab we are getting 1 mg; 1,5 mg... Is that possible o we have to beggin to make questions about our nanodrop?

26 February 2018 6,110 2 View

Has anybody purified pet28 produced in 50ml of LB using MIDI?

The pet28 is a very low copy plasmid. I cant get a pellet in isopropanol step. I have to use a mini spin kit Qiagen a to do fraction of 10 ml.

26 January 2018 5,285 3 View

How can I analize agreggation of a protein with a gel filtration cromatografy?

I wanna know how much homodimeric form or heterodimeric form there are, and I want to separe their.

13 July 2017 5,913 5 View