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Questions related from Iñigo Lobregat
I want to identify if certain phosphorylation sites are conserved for protein X across humans and yeast. I know from MS data that there are 4 phospho sites in Human protein X. In order to identify...
11 December 2015 826 3 View
I am producing a yeast line with an allele mutated. The protocol to follow will be this one: Obtain gDNA --> clone the desired gene to a plasmid --> quickchange mutagenesis --> introduce...
30 October 2015 9,658 2 View
I want to check conserved post-translation sites (in my case acetylation sites) in a given protein between human and yeast sequences. I guess there are bioinformatic tools available, I would thank...
29 October 2015 8,682 4 View
Hey, I am designing some primers for a cloning. It turns out that I want to use a hexaglycine linker in order to produce fusion proteins, and in order to do so I have to use a primer that would...
12 August 2015 9,624 6 View
I am interested on obtaining native chromatin from Hela cells. The ultimate goal is to do mass spectrometry in the purified fragments in order to check the proteome found there. Yet I am...
24 July 2015 648 3 View
Hi, I have left restriction digested and rSAP treated DNA (originally plasmid of 7Kb, now linear of same length) at room temperature for a week. Will it be alright to use, or rather degraded? (or...
04 July 2015 614 4 View
I am trying to clone a 700 bp fragment to a 7 kb vector. My insert has ClaI restriction site in both ends and my vector has 1 ClaI accordingly. I have been told to use CIP in the linearized vector...
25 June 2015 2,277 4 View
Double Thymidine block is used to synchronize cells. With this system it is possible to obtain cells arrested in the G1 / S phase that will follow cell cycle in a synchronized fashion for more or...
17 May 2015 1,428 4 View
