Iñigo Lobregat

5 Questions 5 Answers 0 Followers

Questions related from Iñigo Lobregat

Conserved PTM sites?

I want to identify if certain phosphorylation sites are conserved for protein X across humans and yeast. I know from MS data that there are 4 phospho sites in Human protein X. In order to identify...

11 December 2015 776 3 View

Would cut the "post stop codon" sequence in my genes affect the transcription ending and mRNA stability?

I am producing a yeast line with an allele mutated. The protocol to follow will be this one: Obtain gDNA --> clone the desired gene to a plasmid --> quickchange mutagenesis --> introduce...

30 October 2015 9,631 2 View

Is this primer safe?

Hey, I am designing some primers for a cloning. It turns out that I want to use a hexaglycine linker in order to produce fusion proteins, and in order to do so I have to use a primer that would...

12 August 2015 9,608 6 View

Is linear DNA stable at room temperature?

Hi, I have left restriction digested and rSAP treated DNA (originally plasmid of 7Kb, now linear of same length) at room temperature for a week. Will it be alright to use, or rather degraded? (or...

04 July 2015 591 4 View

How can I keep Hela cells without undergoing mitosis for 24h?

Double Thymidine block is used to synchronize cells. With this system it is possible to obtain cells arrested in the G1 / S phase that will follow cell cycle in a synchronized fashion for more or...

17 May 2015 1,402 4 View