Why I can't get stable signal of Bodipy 581/591 when I want to detect of lipid peroxidation caused by PEF in Saccharomyces cerevisiea?

Any suggestion what I should change? I'm trying of 2kV/cm ; 4 kV/cm; 10 kV/cm PEF treatment. What concentration of cells I should use?

24 April 2018 6,400 3 View