Imran Ullah

5 Questions 5 Answers 0 Followers

Questions related from Imran Ullah

What is the most efficient method for RNA isolation as well as immunofluorescence analysis of spheroid/ organoids embeded in matirgel?

I am facing problem in isolation of protein/RNA from newly generated organoid/spheroids embeded in matrigel. I know about the use of matrigel recovery solution (BD); however, looking forward to...

08 February 2019 803 11 View

Does anyone have experience of porcine Pancreatic beta cells karyotyping? Looking forward to hear expert opinions?

I am working on differentiation of porcine ear fibroblast to Pancreatic beta cells. Our differentiation protocol is very successfull and we had generated mature pancreatic beta cells. The problem...

11 October 2018 312 2 View

Anyone have experience of data analysis (OD) obtained from Mercodia C-peptide ELISA kit?

I have querry about the data analysis (basically OD) from Mercodia c-peptide ELISA kit. They recommended the linear regression plot between calibrators (standards) and c-peptide concentration;...

21 August 2018 5,582 1 View

Can somebody help me how to subculture pacreatic cells (insulin positive cells) generated from pluriptent stem cells (MSCs).

Hello, I am working on generation of pancreatic cells (insulin secreting cells) from mesenchymal stem cells. I am almost successfull but need to know someone personal experience or good protocol...

16 August 2017 5,171 2 View

When designing primers for qPCR, which sequence is better to consider, CDS or mRNA?

I am designing primer for real time pcr but i have some confusion in selecting the sequence. Is it the mRNA sequence or CDS of respective gene? or we can select anyone?.

16 April 2015 2,499 7 View