Imran Ullah

3 Questions 3 Answers 0 Followers

Questions related from Imran Ullah

What is the most efficient method for RNA isolation as well as immunofluorescence analysis of spheroid/ organoids embeded in matirgel?

I am facing problem in isolation of protein/RNA from newly generated organoid/spheroids embeded in matrigel. I know about the use of matrigel recovery solution (BD); however, looking forward to...

08 February 2019 744 11 View

Anyone have experience of data analysis (OD) obtained from Mercodia C-peptide ELISA kit?

I have querry about the data analysis (basically OD) from Mercodia c-peptide ELISA kit. They recommended the linear regression plot between calibrators (standards) and c-peptide concentration;...

21 August 2018 5,546 1 View

When designing primers for qPCR, which sequence is better to consider, CDS or mRNA?

I am designing primer for real time pcr but i have some confusion in selecting the sequence. Is it the mRNA sequence or CDS of respective gene? or we can select anyone?.

16 April 2015 2,414 7 View