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Questions related from Imdad Ullah Khan
how long the serum samples can be stored at -80C? i have intended to use them for the detection of cytokines (IL10) through ELIZA (commercially available).
13 February 2018 7,517 11 View
how long the DMEM can be stored at 4C after reconstituting with 10% FBS and 1% PS ?
22 September 2017 2,506 2 View
what is the best %age of Gel used to separate the low molecular weight proteins i.e 10-15 KD in western blot procedures?
09 November 2016 9,146 2 View
with reference to various research articles; relating to various spinal cord injury experiments, the spinal cord is preferred to be harvested from live animal (mouse, dog) for the purpose of...
08 November 2016 7,902 12 View
I am getting multiple bands for TNF-alpha and IL-6 on western blot membrane which make me difficult to search for the desire band. is it due to problem with the primary antibody or improper washing?
23 August 2016 1,859 9 View
i am using stripping buffer for 1hr to remove the previous bands but still the previous band are visible. is it possible to completely remove the previous bands with stripping buffer ? .
04 August 2016 2,497 3 View
