Ilze Olivi

4 Questions 1 Answers 0 Followers

Questions related from Ilze Olivi

CRISPR partial gene reduction but protein is still detectable, wth?

Hello everyone, I'm helping an undergrad student who tried to knockout the DUSP2 gene in the PDAC cell line (Mia Paca-2). The RT-qPCR result showed a 60% reduction of gene expression, but the...

10 October 2019 2,253 2 View

RNA phosphatase inhibitor?

I need an RNA phosphatase inhibitor but I'm finding only ser/thr phosphatase inhibitors. Can anyone help me?

11 November 2018 599 1 View

How to do 3D culture for pancreatic ductal adenocarcinoma cells?

I want to stablish 3D cell culture from cell line and from KPC mice, but I can´t find the "ideal protocol". I want to do 3D cells to modulate miRNA expression, and maybe do some viability,...

08 August 2018 10,089 0 View

How can I calculate or find the effect size for sample size determination?

I need to calculate sample size of mice to calculate the difference between miRNA (2 groups). But I can`t find the effect size of it! Anyone??

03 March 2018 6,951 4 View