8 Questions 17 Answers 0 Followers
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Dear All, I am expressing a GST fusion protein in Bacterial cells and on purification of the protein, there are a lot of contaminating nucleic acids. I tried various approaches to get rid off the...
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Dear All I want to label in-vitro transcribed RNA with Cy5 dye on one end, what could be the best possible way to do that. For DNA, I am simply labelling one of my Primer with Cy5. If I label Cy5...
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Dear All, I need to amplify a region of greater than 350bps and I know that usually we do not go beyond 200-300 bp while doing qPCRs. The problem that I have is that my reverse primer is in vector...
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I am establishing a monoclonal cell line transfected with my transgene. I have picked up the clones which are growing in 48-well plates. However, I see that there are some needle like structures...
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Dear All, I am trying to establish stable cell lines expressing my transgene. The transfections seemed to have worked fine and I had picked the clones few weeks back which were first growing in...
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Dear All, What is the best way of picking the positive clones while establishing a monoclonal cell line expressing your desired transgene. I am currently doing it with a P-20 pipette under a light...
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Dear all, I am trying to PCR some fragments using Genomic DNA. These fragments are to be cloned using Gibson Assembly, hence, the primers that I am using are overlapping with plasmid sequences and...
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