Ian Lidbury

2 Questions 20 Answers 0 Followers

Questions related from Ian Lidbury

What is the feasibility for cloning a four gene operon (total 4kb) into an expression vector and getting the subsequent expression of proteins?

I am thinking of cloning a four gene cluster operon and inserting into pET28a. Has anyone had success with this size product and type of gene arrangement. All four genes are subunits of a single...

05 May 2014 3,642 2 View

Is this the correct formula for calculating Miller Units?

I am currently running some lacZ reporter assays and am just confirming if this is the correct formula to use to calculate Miller Units : The formula is: OD(420) - [1.75 *...

06 June 2013 3,987 4 View