Ian Wright

3 Questions 4 Answers 0 Followers

Questions related from Ian Wright

Recombinant Interleukin 6 western blot, non specific bands?

Hi I've been trying to produce some recombinant human Interleukin 6. I sub-cloned the gene for human IL6 into a Promega T7 Flexi vector (native gene no tag yet) and confirmed sequencing and...

10 December 2020 2,532 4 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

10 December 2020 2,384 8 View

Does anyone know the dyes used in Promega GoTaq G2 Green Flexi buffer?

Or is there another dye that can be used without interfering with the PCR reaction? I would like to be able to make my own X5 PCR buffer and load it straight to the gel after the PCR without...

01 May 2015 4,092 3 View