Ian N Oyaro

4 Questions 5 Answers 0 Followers

Questions related from Ian N Oyaro

Anyone with experience in staining PBMC for chemokine receptors?

I am working on characterizing cellular subtypes in PBMC. I never seem to quite get a good positive population for CXCR5, despite conducting titrations for the same. Is there a special way of...

24 April 2017 5,577 4 View

Is there a way of increasing the number of the query matches in a CLC Bio blast output?

I have been blasting against NCBI using CLC Bio genomic workbench.Since my samples have more than one query sequence, the default view of the summary table i see is whereby only the description of...

22 April 2015 8,961 2 View

Can someone share the protocol for Bacterial DNA extraction from a patients' sample, more preferably plasma or csf?

I am trying to optimize the extraction for downstream applications- amplification (conventional pcr) and sequencing

30 September 2014 6,502 2 View

For optimal Bacterial DNA extraction, is it really necessary to do an overnight proteinase K digestion coupled with lysozyme enzyme?

My research entails extracting Bacterial DNA from patients' samples (plasma and csf) as I explore to use molecular techniques to identify bacteria indicated in non-traumatic coma. As most bacteria...

30 September 2014 4,318 12 View