Hyunsoo Cho

2 Questions 6 Answers 0 Followers

Questions related from Hyunsoo Cho

Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

14 December 2020 9,301 9 View

For recombinant protein expression, do I need antibiotics for large culture?

Hi, I am currently using BL21(DE3)PLySs for protein expression. In our lab, we are using selective antibiotics for both inoculation(small culture, a.k.a over night seed culture) and scale up(Large...

07 April 2020 2,738 9 View