Hyunsoo Cho

3 Questions 6 Answers 0 Followers

Questions related from Hyunsoo Cho

Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

14 December 2020 9,487 9 View

Does Hitrap Q column needs to be activated(recharged)?

Hi guys, I am currently using Hitrap Q of GE's. I read that some people use 0.5N NaOH to activate Q resin(to charge negative). Is it necessary? I read GE's catalog and it did not mention about it...

08 April 2020 9,717 0 View

For recombinant protein expression, do I need antibiotics for large culture?

Hi, I am currently using BL21(DE3)PLySs for protein expression. In our lab, we are using selective antibiotics for both inoculation(small culture, a.k.a over night seed culture) and scale up(Large...

07 April 2020 2,826 9 View