Huong Ha

13 Questions 60 Answers 0 Followers

Questions related from Huong Ha

Can anyone please help with primer dimer issue in sybr green qPCR reaction?

Dear everyone, May I please get your help with primer dimer issue in sybr green qPCR reaction? Our lab is working on developing diagnostic methods based on cell-free RNA from plasma. We are...

24 March 2020 9,583 5 View

How to increase AMPAR mEPSCs frequency from hippocampal neuronal culture?

Hi all, Do you know of any strategy to increase AMPAR mEPSCs frequency for cultured hippocampal neurons? Methods on the side of culturing and/or of recording environment are much...

29 November 2016 6,404 5 View

Experience with paired recording/ local bipolar electrode stimulation in cultured neurons?

Hi all, I am trying to record evoked AMPA EPSC from embryonic rat hippocampal cultures. If you have experience using local platinum/tungsten bipolar electrodes or paired recording, would you...

20 November 2016 4,610 10 View

Could someone please help me with cloning, particularly for shRNAs?

Hi everyone, my labmate and I have been trying to clone a few shRNA constructs in the past 2 months. We encountered quite a few issues and only successfully cloned 2 out of 18 constructs so far. I...

07 September 2016 8,723 3 View

Does Cs internal create a drop in membrane potential? And what is your experience with this?

Hi all, I am doing some voltage clamp experiment to look at AMPAR mEPSC in cultured hippocampal neurons. And at the moment, I think I nailed down a good pair of internal (CsGlu) and external...

28 March 2015 9,126 2 View

How to increase the frequency/amplitude of AMPAR mediated mini EPSCs in culture neurons?

Hi all, I am interested in AMPAR mediated mini EPSCs in hippocampal neurons. My current recording configuration (hibernate E as bath solution and Cs gluconate as internal) seem to allow me to...

17 March 2015 7,268 20 View

What bath solutions do you prefer to use for cell culture recording?

Hi all, Would you mind sharing some of your experience with hippocampal cell culture recording? I have been testing out Hibernate E (a defined media from Brain Bit) and tyrodes (similar formula...

10 March 2015 8,847 25 View

How do I explain round circles appearing in my hippocampal neuronal culture?

Hi all, I just moved to a new lab and started cell cultures again. We are culturing hippocampal neurons from E18 rat embryos. The cells seem to mature at normal rate and I can see synaptic...

01 March 2015 3,072 11 View

How do I fix the drift of pipettes to do whole-cell patch clamp?

Hi all, I am having some difficulty with fixing the pipette drift in my rig. I am wondering if you could give me some advice on this as it looked like I ran out of ideas to try. - I am trying to...

14 October 2014 6,768 19 View

Can anybody help me to fix this problem with multiclamp 700B?

Hi all, I am seeking for your help with the multiclamp 700B. I am using it for patch clamp in hippocampal neurons. And sometimes when I did pipette offset, the holding voltage just suddenly...

12 October 2014 6,468 16 View

What osmometer should I use for electrophysiology? And how frequently do you make your patching solutions?

Hi guys, I am a beginner patch clamper and the only one that does ephys in my lab. Hence, we do not have all of the necessary equipment including an osmometer. I am currently going to another lab...

06 October 2014 3,877 18 View

Why are our hippocampal neurons that loaded well with some dyes not loading well when they have mcherry tag?

Hi all, I am trying to image zinc in our hippocampal neurons around DIV 12 - 21. I tried both New Port Green and Fluozin-3 (from 1 - 5 uM, load 30 min - 1 hour at RT, wash for 30 min) and the...

26 August 2014 5,804 3 View

Can anyone help with recording AMPAR mediated mini EPSC in dissociated hippocampal cultured?

I just started doing electrophysiology since last year and now interest in learning about the zinc sensitivity of AMPAR mediated mini EPSC. For this goal, I am trying to record from dissociated...

10 April 2014 9,138 19 View