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Questions related from Huihun Jung
In my lab, we have Nicolet6700 FTIR. We replaced a laser on the machine recently, and my FTIR signal is not as strong as before. I found the error messages (shown below and in the attached...
16 August 2022 9,160 0 View
I have used 5mC (5' methyl cytosine) to impair Acc65I recognition sites randomly in linearized repetitive sequences, but I want to build up with methylated adenine nomoners for PCR amplification....
10 January 2019 9,920 0 View
Hi, I am producing proteins from e. coli (BL21-DE3 strain, pET14b vector, IPTG induction). Usually, I had enough amount of proteins (50mg of proteins from 1L LB media of 100mg/L ampicillin...
09 June 2016 5,696 4 View
Hi all, I am working on designing insoluble proteins and their behaviors. What I want to know is "Can I use the dynamic light scattering (DLS) to see the aggregation properties even if my proteins...
08 June 2016 9,822 7 View
I am wondering about the rough calculation for linear polypeptide chain length in nanometer. For example, if I have a linear chain of 10 amino acid polypeptide, then what nano meter length they...
14 April 2016 4,396 8 View
I am running an insoluble protein in SDS gel to check its size. I prepared 25mg/mL stock solution and add 1-10 uL of it to my loading buffer but I cannot get the image. Does anyone know how much...
22 December 2015 8,700 6 View
I am using a pET14b vector (with ampicillin resistance) to express and purify several recombinant proteins but I always have problems on expression of recombinant protein production larger than...
04 August 2015 715 2 View
